They can be detected using Janus green B or enzimo-histochemistry.
2.
The indicator Janus Green B changes colour according to the amount of oxygen present.
3.
Together with Janus Green B, it is used to stain embryonal tissues and supravital staining of blood.
4.
"' Janus Green B "'is a basic dye and vital stain used in histology.
5.
The first high-resolution electron micrographs appeared in 1952, replacing the Janus Green stains as the preferred way of visualising the mitochondria.
6.
Some of the dyes used are methylene blue, prussian blue, brilliant cresyl blue, Toluidine Blue O, Basic Blue 3, Methylene Green, Taylor's Blue, Janus Green B, Meldola's Blue, Thionin, Nile Blue, and Celestine Blue.
7.
Besides his role in the formulation of the famous Michaelis Menten equation ( 1913 ) he discovered Janus green as a supravital stain for mitochondria and the Michaelis Gutmann body in urinary tract infections ( 1902 ) and found that thioglycolic acid could dissolve keratin, a discovery that would come to have several implications in the cosmetic industry, including the permanent wave.
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